With an optional condenser, objectives and sliders dedicated for phase contrast and darkfield observation, even an unstained specimen that is colorless and transparent can be observed with clear contrast.
Both of these systems make use of an ultra-long working distance objective lens, which makes possible features not available in previous microscopes and, as such, are unique microscope systems with distinctive operability features.
Silicone immersion objectives can be designed with a larger numerical aperture(NA) than water immersion objectives, increasing image resolution and brightness.
In addition, silicone immersion objectives have a higher numerical aperture(NA) than water immersion objective lenses, so researchers can obtain higher resolution images deeper inside cleared tissues compared to what can be obtained using a water immersion objective lens.
Leica Microsystems will utilize the Sensofar technology plus Leica Microsystems' high-end optical objectives to provide a new and complete solution for sophisticated optical profiling and 3D surface metrology with a vertical accuracy down to 0.1 nm.
The pair of objectives can be two 50x SLWDs for measuring PSI tilted surfaces, such as silicon wafers, at 45 degree without colliding with the objective..
For low magnification objectives, Focus Variation is more suitable for the measurement of the form and waviness components of a surface more than its texture.
With 13 inches(330 millimeters) of space below the objectives, there is easy accessibility to part areas that simply can't be reached with other types of profilometers.
Because the objectives cover a field of view larger than 1 cm, imaging of embryos during late stages of development and capturing the dynamics of cell populations in whole organs are possible.
The NEXUS 3400FA is equipped with a 6-position motorized turret, with 3 indenter positions, a laser positioning system and 2 LWD objectives with ring lights.
The various combinations of objectives and zoom lenses allow you to select and prioritize what's more important for the study at hand, including measurement field of view, lateral resolution, or surface opacity slope.
For instance, Interferometry provides the highest vertical resolution independently of the numerical aperture of the objective, but I has the drawback of being highly sensitive to vibration and requires large amount of acquired images to extract the areal information.
Optical System The IXplore SpinSR system is composed of an objective, imaging lens, optical system for magnification, confocal spinning disk, and an sCMOS camera Figure 8.
Because the mercury surface is always keeping a horizontal plane even if the telescope itself declines, the light of the star which comes from the same direction focuses into an image always at the same point on the focal plane beneath the objective.
By employing the DP80's monochrome CCD mode in conjunction with the 20X high NA phase-contrast objective lens(UCPLFLN20XPH), Nanog-GFP expressed in mouse iPS cells was efficiently detected at a lower light intensity, as illustrated by the images below.
If there is a material absorbs both an excitation laser and a probe laser through an objective lens, the light energy except the energy absorbed by the sample is all released to the solvent as thermal energy, and the phenomenon produces the increase in temperature.
Spherical aberration, which occurs with oil or water immersion objectives due to a refractive-index mismatch with biological samples, is reduced in silicone immersion objectives, allowing researchers to achieve deep, high-contrast fluorescence imaging at a greater depth.
As an example of live-cell imaging using a 30' silicone immersion objective, UPLSAPO30XS, the process of Arabidopsis embryogenesis was stably observed in real time for 67 hours from the early embryo(4-cell stage) to the late embryo(Movie).
For the measurement of large areas while keeping few nm of system noise, it is needed to use of high numerical aperture objectives, move the sample in the XY plane and stitch several fields together to cover the required surface.
Thanks to a short-wavelength 405nm laser light, a higher-aperture(high-N.A.) objective lenses and confocal optics, high X-Y resolution is available so that objects not visible with the optical microscope can be observed in clear images.
English
中文
عربى
Български
বাংলা
Český
Dansk
Deutsch
Ελληνικά
Español
Suomi
Français
עִברִית
हिंदी
Hrvatski
Magyar
Bahasa indonesia
Italiano
Қазақ
한국어
മലയാളം
मराठी
Bahasa malay
Nederlands
Norsk
Polski
Português
Română
Русский
Slovenský
Slovenski
Српски
Svenska
தமிழ்
తెలుగు
ไทย
Tagalog
Turkce
Українська
اردو
Tiếng việt