英語 での Objective lens の使用例とその 日本語 への翻訳
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To increase resolution power of fluorescence microscopy, immersion oil, which has a high refractive index(RI), is normally placed between the glass coverslip and objective lens.
Both of these systems make use of an ultra-long working distance objective lens, which makes possible features not available in previous microscopes and, as such, are unique microscope systems with distinctive operability features.
Application note“Fluorescence, phase-contrast, and bioluminescence imaging of live cells incubated in plastic bottom dishes using a 20X high numerical aperture(NA) phase contrast objective lens”.
In addition, silicone immersion objectives have a higher numerical aperture(NA) than water immersion objective lenses, so researchers can obtain higher resolution images deeper inside cleared tissues compared to what can be obtained using a water immersion objective lens.
Limited length with 160 tube length objective The difference: 160 tube length objective lens can not change its optical distance, and infinity objective lens can be added to other devices;
The JSM-7500F features an optical system that includes a semi-in-lens type objective lens, which can collimate the electron beam even at low accelerating voltages.
By employing the DP80's monochrome CCD mode in conjunction with the 20X high NA phase-contrast objective lens(UCPLFLN20XPH), Nanog-GFP expressed in mouse iPS cells was efficiently detected at a lower light intensity, as illustrated by the images below.
If there is a material absorbs both an excitation laser and a probe laser through an objective lens, the light energy except the energy absorbed by the sample is all released to the solvent as thermal energy, and the phenomenon produces the increase in temperature.
If objective lens 20x is used, pitch of less than 0.05 mm can be read easily, but there may be limitations in actual use, such as a much narrower field of vision when compared with the standard objective lens 10x.
The spatial resolution of the Raman microscope is about 300 nm at the theoretical limit when using a 532 nm laser and a 0.90 NA objective lens, so the scanning accuracy(100 nm) of the motorized stage can be said to be sufficient.
In this application note, we demonstrate how Olympus' 20X high NA phase contrast objective lens enables vivid fluorescence and phase-contrast observation of stem cells in a plastic bottom dish as well as an example of bioluminescence imaging observation using the same objective lens.
Optical magnification(magnification of the objective lens)×(magnification of the lens) In the microscope of the below picture, the magnification of the objective lens is 10× magnification× 2 times the magnification of the lens body, the optical magnification is"20 times.
Olympus then developed a long barrel(LB) objective lens series(1x-100x oil) with a focal length of 45 mm and a mechanical tube length of 160 mm that could be used for everything from regular brightfield observations to polarization, fluorescence, and phase contrast microscopy.
Please be advised that the part number of the objective lens(Long working distance objective lens, Near infrared objective lens, Near ultraviolet objective lens, Ultraviolet objective lens, 3-wavelength objective lens) will be changed for the purpose of the standardization of the part number.
The DFPlan series combines high-NA resolving power and working comfort with"distortion-free" optical design for exceptionally flat imaging, and includes a 2x objective lens with an NA of 0.2 for high-resolution imaging at 600 linepair per millimeter.
In the observation using dichroic mirror fluorescence microscopy and polarization elimination by coating on the mirror surface, also the polarization elimination effect by using high aperture objective lens still have problem. The problem is the dynamic range of the observed fluorescence anisotropy is small.
Above the objective lens 8, typically micro-plate 9 which one to several tens μL sample container or well 10 the sample solution is dispensed in are arranged is disposed, it is emitted from the objective lens 8 laser beam is focused in the sample solution in the sample container or well 10, the strong areas of the light intensity(excitation region).
If the critical angle prism is adjusted so that it makes the exact critical angle to parallel rays, that is, the angle at which the incident rays are totally reflected, the amounts of light on either sides of the optical axis in the prism are unbalanced in proportion to the distance between objective lens and target surface.
Nanog-GFP expressed mouse iPS cells Photographic conditions: Specimen: Mouse iPS cells Fluorescence label: Nanog-GFP Incubation chamber: Plastic bottom dish Objective lens: High NA phase-contrast objective lens UCPLFLN20XPH CCD camera: DP80 digital microscope camera Microscope: Inverted research microscope IX73 Application: High-performance bioluminescence imaging Olympus' 20X high NA phase-contrast objective lens(UCPLFLN20XPH) achieves outstanding performance for demanding applications such as bioluminescence imaging with plastic bottom dishes.
High-Power Objective Lens.